Teacher Profiles

MIYAUCHI, Keisuke Professor (Environmental Bioengineering)

Career

performance

List of peer-reviewed papers

• Nagata, Y., R. Ohtomo, K. Miyauchi, M. Fukuda, K. Yano, and M. Takagi. 1994. Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis. J Bacteriol 176:3117-25.
• Nagata, Y., K. Miyauchi, J. Damborsky, K. Manova, A. Ansorgova, and M. Takagi. 1997. Purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. Appl Environ Microbiol 63:3707-10.
• Miyauchi, K., SK Suh, Y. Nagata, and M. Takagi. 1998. Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by Sphingomonas paucimobilis. J Bacteriol 180:1354-9.
• Miyauchi, K., Y. Adachi, Y. Nagata, and M. Takagi. 1999. Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis. J Bacteriol 181:6712-9.
• Nagata, Y., A. Futamura, K. Miyauchi, and M. Takagi. 1999. Two different types of dehalogenases, LinA and LinB, involved in gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing. J Bacteriol 181:5409-13.
• ​FEBS Lett. 459:395-8.
• Nagata, Y., K. Miyauchi, and M. Takagi. 1999. Complete analysis of genes and enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. J Ind Microbiol Biotechnol 23:380-390.
• Kitagawa, W., K. Miyauchi, E. Masai, and M. Fukuda. 2001. Cloning and characterization of benzoate catabolic genes in the gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. J Bacteriol 183:6598-606.
• Kitagawa, W., S. Takami, K. Miyauchi, E. Masai, Y. Kamagata, JM Tiedje, and M. Fukuda. 2002. Novel 2,4-dichlorophenoxyacetic acid degradation genes from oligotrophic Bradyrhizobium sp. strain HW13 isolated from a pristine environment. J Bacteriol 184:509-18.
• Lee, HS, K. Miyauchi, Y. Nagata, R. Fukuda, S. Sasagawa, H. Endoh, S. Kato, H. Horiuchi, M. Takagi, and A. Ohta. 2002. Employment of the human estrogen receptor beta ligand-binding domain and co-activator SRC1 nuclear receptor-binding domain for the construction of a yeast two-hybrid detection system for endocrine disrupters. J Biochem (Tokyo) 131:399-405.
• Miyauchi, K., HS Lee, M. Fukuda, M. Takagi, and Y. Nagata. 2002. Cloning and characterization of linR, involved in regulation of the downstream pathway for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. Appl Environ Microbiol 68:1803-7.
• Sakai, M., K. Miyauchi, N. Kato, E. Masai, and M. Fukuda. 2003. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol 69:427-33.
• Masai, E., A. Ichimura, Y. Sato, K. Miyauchi, Y. Katayama, and M. Fukuda. 2003. Roles of the enantioselective glutathione S-transferases in cleavage of beta-aryl ether. J Bacteriol 185:1768-75.
• Hara, H., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2003. Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6. J Bacteriol 185:41-50.
• Masai, E., M. Sasaki, Y. Minakawa, T. Abe, T. Sonoki, K. Miyauchi, Y. Katayama, and M. Fukuda. 2004. A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate. J Bacteriol 186:2757-65.
• Takeda, H., N. Hara, M. Sakai, A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Biphenyl-inducible promoters in a polychlorinated biphenyl-degrading bacterium, Rhodococcus sp. RHA1. Biosci Biotechnol Biochem 68:1249-58.
• Takeda, H., A. Yamada, K. Miyauchi, E. Masai, and M. Fukuda. 2004. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J Bacteriol 186:2134-46.
• Warren, R., WW Hsiao, H. Kudo, M. Myhre, M. Dosanjh, A. Petrescu, H. Kobayashi, S. Shimizu, K. Miyauchi, E. Masai, G. Yang, JM Stott, JE Schein, H. Shin, J. Khattra, D. Smailus, YS Butterfield, A. Siddiqui, R. Holt, MA Marra, SJ Jones, WW Mohn, FS Brinkman, M. Fukuda, J. Davies, and LD Eltis. 2004. Functional characterization of a catabolic plasmid from polychlorinated- biphenyl-degrading Rhodococcus sp. strain RHA1. J Bacteriol 186:7783-95.
• Kasai, D., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2004. Characterization of the 3-O-methylgallate dioxygenase gene and evidence of multiple 3-O-methylgallate catabolic pathways in Sphingomonas paucimobilis SYK-6. J Bacteriol 186:4951-9.
• Sakai, M, E. Masai, K. Sugiyama, C. Takahashi, A. Yamada, K. Miyauchi, and M. Fukuda. 2004. Characterization of a second 2,3-dihydro-2.3-dihydroxybiphenyl dehydrogenase gene for the polychlorinated bipenyl degrader Rhodococcus sp. strain RHA1. J Environ. Biotech. 4 (1):41-47.
• Miyauchi, K., M. Fukuda, M. Tsuda, M. Takagi, and Y. Nagata. 2005. Identification of insertion sequence from a gamma-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26. Biosci Biotechnol Biochem 69:216-9.
• Kasai, D., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6. J Bacteriol 187:5067-74.
• Peng, X., E. Masai, D. Kasai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. A second 5-carboxyvanillate decarboxylase gene, ligW2, is important for lignin-related biphenyl catabolism in Sphingomonas paucimobilis SYK-6. Appl Environ Microbiol 71:5014-21.
• Abe, T., E. Masai, K. Miyauchi, Y. Katayama, and M. Fukuda. 2005. A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6. J Bacteriol 187:2030-7.
• Endo, R., M. Kamakura, K. Miyauchi, M. Fukuda, Y. Ohtsubo, M. Tsuda, and Y. Nagata. 2005. Identification and characterization of genes involved in the downstream degradation pathway of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis UT26. J Bacteriol 187:847-53.
• Sasoh, M., E. Masai, S. Ishibashi, H. Hara, N. Kamimura, K. Miyauchi, and M. Fukuda. 2006. Characterization of the terephthalate degradation genes of Comamonas sp. strain E6. Appl Environ Microbiol 72:1825-32.
• Iwasaki T, Miyauchi K, Masai E, Fukuda M. 2006. Multiple-subunit genes of the aromatic-ring-hydroxylating dioxygenase play an active role in biphenyl and polychlorinated biphenyl degradation in Rhodococcus sp. strain RHA1. Appl Environ Microbiol 72:5396-402.
• McLeod MP, Warren RL, Hsiao WW, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, Dosanjh M, Hara H, Petrescu A, Morin RD, Yang G, Stott JM, Schein JE, Shin H, Smailus D, Siddiqui AS, Marra MA, Jones SJ, Holt R, Brinkman FS, Miyauchi K, Fukuda M, Davies JE, Mohn WW, Eltis LD. 2006. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Natl Acad Sci US A. 2006 103:15582-7.
• Iwasaki T, Takeda H, Miyauchi K, Yamada T, Masai E, Fukuda M. 2007. Characterization of two biphenyl dioxygenases for biphenyl/PCB degradation in A PCB degrader, Rhodococcus sp. strain RHA1. Biosci Biotechnol Biochem. 71: 993-1002.
• Yamada T, Miyauchi K, Ueda H, Ueda Y, Sugawara H, Nakai Y, Endo G. 2007. Composting cattle dung wastes by using a hyperthermophilic pre-treatment process: characterization by physicochemical and molecular biological analysis. J Biosci Bioeng. 104:408-15.
• Yamada, T., Miyauchi, K., Ueda, Y., Ueda, H., Endo, G. 2007. Development of environmentally friendly compost production technology necessary for the circular use of organic resources. Journal of the Japan Society for Environmental Biotechnology, 7: 111-117.
• Miyauchi K, Sukda P, Nishida T, Ito E, Matsumoto Y, Masai E, Fukuda M. 2008. Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes. J Biosci Bioeng. 105:628-35.
• Yamada T, Suzuki A, Ueda H, Ueda Y, Miyauchi K, Endo G. 2008. Successions of bacterial community in composting cow dung wastes with or without hyperthermophilic pre-treatment. Appl Microbiol Biotechnol. 81:771-81.
• Sukda P, Gouda N, Ito E, Miyauchi K, Masai E, Fukuda M. 2009. Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Nocardioides sp. Strain DF412. Biosci Biotechnol Biochem. 73:508-16
• Takeda, H., Shimodaira, J., Yukawa, K., Hara, N., Kasai, D., Miyauchi, K., Masai, E., Fukuda, M. 2010. Dual Two-Component Regulatory Systems Are Involved in Aromatic Compound Degradation in a Polychlorinated-Biphenyl Degrader, Rhodococcus jostii RHA1. J Bacteriol. 192:4741-4751
• Miyahara, M., Kim, SW, Fushinobu, S., Takaki, K., Yamada, T., Watanabe, A., Miyauchi, K., Endo, G., Wakagi, T., Shoun, H. 2010. Potential of Aerobic Denitrification by Pseudomonas stutzeri TR2 To Reduce Nitrous Oxide Emissions from Wastewater Treatment Plants. Appl Environ Microbiol. 76:4619-4625
• Ohmori T, Morita H, Tanaka M, Tomoi M, Miyauchi K, Kasai D, Furukawa K, Masai E, Fukuda M. 2011. Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1. Biosci Biotechnol Biochem. 75:26-33.
• Araki N, Niikura Y, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011. Glucose-mediated transcriptional repression of PCB/biphenyl catabolic genes in Rhodococcus jostii RHA1. J Mol Microbiol Biotechnol. 20:53-62.
• Araki N, Suzuki T, Miyauchi K, Kasai D, Masai E, Fukuda M. 2011. Identification and Characterization of Uptake Systems for Glucose and Fructose in Rhodococcus jostii RHA1. J Mol Microbiol Biotechnol. 20:125-136.
• Ohmori T, Morita H, Tanaka M, Miyauchi K, Kasai D, Furukawa K, Miyashita K, Ogawa N, Masai E, Fukuda M. 2011. Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways. J Biosci Bioeng. 111:437-442.
• Nguyen AT, Sato Y, Iwasaki T, Miyauchi K, Tokuda M, Kasai D, Masai E, Fukuda M. 2011. Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation system in Janibacter sp. TYM3221. Enzyme Microb Technol. 10:532-539
• Shindo, Erika; Otsubo, Wakako; Ueda, Yuichi; Ueda, Hideyo; Miyauchi, Keisuke; Endo, Ginro. 2011. Diversity and fluctuations of denitrifying bacterial genes involved in the reduction of nitrous oxide generated during the composting process. Journal of Japan Society of Civil Engineers, Series G (Environment). 67:III441-III448.
• Ito, T., Araki, N., Endo, G., Fukuda, M., Miyauchi, K. 2011. Transcriptional repression of degradation genes in PCB-degrading bacteria in the presence of degradation metabolites. Journal of Japan Society of Civil Engineers, Series G (Environment). 67:III485-III493.
• Mei-Fang CHIEN, Ryo NAKAHATA, Tetsuya ONO, Keisuke, MIYAUCHI, Ginro ENDO. 2012. Mercury removal and recovery by immobilized mercury reducing bacterium Bacillus megaterium MB1. Frontiers of Chemical Science and Engineering. 6:192–197
• Miyahara M., Kim SW, Zhou S., Fushinobu S., Yamada T., Ikeda-Ohtsubo W., Watanabe A., Miyauchi K., Endo G., Wakagi T., and Shoun H.. 2012. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-cultures with activated sludge under the denitrifying conditions. Biosci Biotech Biochem. 76:495-500
• Endo, Ginro; Miyauchi, Keisuke; Jian, Meifang; Inoue, Chihiro; Kitajima, Nobuyuki. 2012. Development of plant-based remediation technology for arsenic-contaminated soil caused by earthquakes and tsunamis. Chemical Engineering. 57:437-442
• Shimodaira J, Furusawa Y, Miyazawa Y, Kasai D, Miyauchi K, Masai E, Fukuda M. 2012. The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter. J Biosci Bioeng. 113:279-85.
• Iino T, Wang Y, Miyauchi K, Kasai D, Masai E, Fujii T, Ogawa N, Fukuda M. 2012. Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil. Appl Environ Microbiol. 78:6954-62.
• Ikeda-Ohtsubo W, Miyahara M, Kim SW, Yamada T, Matsuoka M, Watanabe A, Fushinobu S, Wakagi T, Shoun H, Miyauchi K, Endo G. 2013. Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2. J Biosci Bioeng. 115:37-42.
• Iino T, Miyauchi K, Kasai D, Masai E, Fukuda M. 2013. Characterization of nitrate and nitrite utilization system in Rhodococcus jostii RHA1. J Biosci Bioeng. 115:600-6
• Thi Nguyen PA, Thi Trinh TH, Fukumitsu Y, Shimodaira J, Miyauchi K, Tokuda M, Kasai D,Masai E, Fukuda M. 2013. Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation in Janibacter sp. TYM3221. J Biosci Bioeng. 116:91-100
• Jian Meifang, Miyauchi Keisuke, Inoue Chihiro, Kitajima Nobuyuki, Endo Ginro. 2013. Soil arsenic concentration in alluvial plains of major rivers in Miyagi Prefecture and the effects of the Tohoku earthquake and tsunami. Journal of Japan Society of Civil Engineers (G series) 69: 19-24.
• Jian Meifang, Obata Kazuki, Huang Yi, Miyauchi Keisuke, Endo Ginro. 2013. A study on the absorption and removal of soil arsenic by arsenic-accumulating plants and arsenite oxidation by soil microorganisms. Journal of Japan Society of Civil Engineers, Series G (Environment). 2013. 69:III9-III15
• Ito, T., Endo, G., Fukuda, M., Miyauchi, K. 2013. Enhancement of the transcriptional activity of degrading genes in PCB-degrading bacteria by introducing catechol-degrading genes. Journal of Japan Society of Civil Engineers, Series G (Environment). 69:III485-III493.
• Yamada T, Araki S, Ikeda-Ohtsubo W, Okamura K, Hiraishi A, Ueda H, Ueda Y, Miyauchi K, Endo G. 2013. Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste. Syst Appl Microbiol. 2013. 36:359-67
• Ikeda-Ohtsubo W., Miyahara M., Yamada T., Watanabe A., Fushinobu S., Wakagi T., Shoun H., Miyauchi K., and Endo G. 2013. Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor. J Biosci Bioeng. 116:722-724
• Ito, T., Endo, G., Fukuda, M., Miyauchi, K. 2014. Effects of intermediate degradation product catechols on the expression of BphS/BphT-controlled genes in the PCB-degrading bacterium Rhodococcus jostii RHA1. J. Environ. Biotech. 14:57-63
• Oyetibo GO, Ishola ST, Ikeda-Ohtsubo W, Miyauchi K, Ilori MO, Endo G. 2015. Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water. Appl Microbiol Biotechnol. 99:3651-7.
• Huang Y, Miyauchi K, Inoue C, Endo G. 2016. Development of suitable hydroponics system for phytoremediation of arsenic contaminated water using an arsenic hyperaccumulator plant Pteris vittata. Biosci Biotech Biochem. 80:614-8.
• Oyetibo GO, Miyauchi K, Suzuki H, Ishikawa S, Endo G. 2016. Extracellular mercury sequestration by exopolymeric substances produced by Yarrowia spp.: Thermodynamics, equilibria, and kinetics studies. J Biosci Bioeng. 122:701-707
• Oyetibo GO, Miyauchi K, Suzuki H, Endo G. 2016. Mercury removal during growth of mercury tolerant and self-aggregating Yarrowia spp. AMB Express. 6:99.
• Huang Y, Miyauchi K, Endo G, Don LD, Manh NC, Inoue C. 2016. Arsenic contamination of groundwater and agricultural soil irrigated with the groundwater in Mekong Delta, Vietnam. Environ Earth Sci. 75:757-763.
• Oyetibo GO, Miyauchi K, Huang Y, Chien MF, Ilori MO, Amund OO, Endo G. 2017. Biotechnological remedies for the estuarine environment polluted with heavy metals and persistent organic pollutants. Int Biodeterio Biodeg. 119:614-625.
• Huang Yi, Miyauchi Keisuke, Mito Mitsuaki, Nakamura Mariko, Naruse Miki, Endo Tsukasa, Inoue Chihiro, Endo Ginro. 2018. Development of a method for purifying arsenic-containing alkaline leachate from construction waste soil treatment sites using arsenic-accumulating plants. Journal of Japan Society of Civil Engineers, Series G (Environment). 74:1-7.

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Non-peer-reviewed papers and others

• Nagata, Y., Fukuda, M., Miyauchi, K., and Takagi, M. Genes and Enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. Molecular Biology of Pseudomonads (ASM press) 58-70 (1996)
• Masao Fukuda, Keisuke Miyauchi, and Eiji Masui. PCB degradation system of Rhodococcus bacteria. Protein, Nucleic Acid, and Enzymes, October 2005, Vol. 50, pp. 1541-7.
• Keisuke Miyauchi, "Exposing the Responsible Parties!" Journal of the Japan Society for Biotechnology, June 2006, Vol. 84, p. 241
• Miyauchi, Keisuke. 2010. Tohoku Gakuin University Biotechnology Research Common Building. Journal of the Japan Society for Biotechnology. 88:685.
• Miyauchi, K., Jian, M., Huang, Y., Otomo, S., Izumi, T., Inoue, C., Kitajima, N., and Endo, G. 2013. Arsenic contamination of soil due to tsunamis and efforts toward its remediation. J Env Biotech. 13:27-30.
• Miyauchi, Keisuke. 2013. Functional analysis of arsenic resistance genes in arsenic-resistant bacteria and remediation of arsenic-contaminated soil using arsenic-accumulating plants. J Japan Soc Extremophiles. 12:50-55.
• Miyauchi, Keisuke. 2013. Phytoremediation of arsenic contamination caused by the Great East Japan Earthquake. Chemistry and Biology. 51:515-517.
• Miyauchi, Keisuke. 2014. Arsenic contamination countermeasures using plants. Journal of the Japan Society on Water Environment. 37:405-408.
• Endo, Ginro; Huang, Yi; Miyauchi, Keisuke. 2015. Development of low-cost purification technology for arsenic-contaminated groundwater using arsenite-oxidizing microorganisms and hydroponics of arsenic-accumulating plants. J Environ Biotech. 5:63-71.
• Otsubo, Wakako; Shindo, Erika; Yamada, Takeshi; Ueda, Hideyo; Ueda, Yuichi; Watanabe, Akira; Miyauchi, Keisuke; Endo, Ginro. 2015. Reducing environmental impact in composting solid waste derived from livestock wastewater treatment systems. J Env Biotech. 15:17-22.
• Huang Yi, Miyauchi K, Inoue C, Endo G. 2016. Development of a hydroponic system for purifying arsenic-contaminated water using arsenic-accumulating plants (translated paper). Research Reports Faculty of Engineering Tohoku Gakuin University. 50:43-48.
• Miyauchi, K., Huang, Y., Sugawara, K., Mito, M., Naruse, M., Nakamura, M., Monma, S., Inoue, C., Endo, G. 2017. Development of arsenic-contaminated water treatment technology using arsenic-accumulating plants. J Env Biotech. 17:35.

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List of research fields

[Molecular microbiology, environmental microbial engineering]

I. Molecular biological analysis of the degradation mechanisms of environmental pollutant-degrading bacteria:

• Enhanced resolution using the genome sequence of the PCB-degrading bacterium Rhodococcus sp. RHA1
• Elucidation of the degradation pathway and transcriptional regulatory mechanism of the dioxin-degrading bacterium Nocardioides sp. DF412.
• Molecular biological analysis of the degradation mechanism of pesticide-degrading bacteria

II. Substance production using microorganisms

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